بررسی اثر ضد میکروبی آب ازونیزه جهت رفع آلودگی محیطی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 جامع امام حسین(ع)

2 میکربشناسی پزشکی- نانوبیوتکنولوژی

3 دانشگاه جامع امام حسین(ع)

چکیده

در این تحقیق، اثر ضد میکروبی آب ازونیزه در خودرو رفع آلودگی محیطی DSM2500-SK بررسی شد. به دنبال وقوع یک حمله بیولوژیک، رفع آلودگی سریع و به موقع موجب جلوگیری از گسترش آلودگی و کاهش دامنه آسیب خواهد شد. بر اساس استانداردهای موجود، اثر ضد میکروبی نسبت به آب ازونیزه خروجی از دستگاه بر روی باکتری‌هایی نظیر سودوموناس آئروژینوزا (باکتری محیطی)، استافیلوکوکوس اورئوس (باکتری گرم مثبت)، اشریشیا کولی (باکتری گرم منفی) و باسیلوس سوبتیلیس (باکتری اسپوردار) مورد بررسی قرار گرفت. میزان حساسیت باکتری‌ها با روش اصلاح شده میکرودایلوشن (Micro Dilution) و از طریق تعیین حداقل غلظت مهار کنندگی و کشندگی آب ازونیزه تعیین و در مرحله بعدی غلظت آب ازونیزه موثر بر باکتری‌ها بررسی شد. افزایش دما، ناخالصی و pH باعث ناپایدار شدن آب ازونیزه می‌شود. نتایج به‌دست آمده نشان داد که حداقل رقت کشنده باکتری توسط ازون باقیمانده در آب با غلظت ppm 3 برای باکتری استافیلوکوکوس اورئوس CFU/mL 104×5/7، برای باکتری سودوموناس آئروژینوزا CFU/mL 105× 5/7، برای باکتری اشریشیا کولی 157O CFU/mL 105×5/7 است و این محلول گندزدا بر روی اسپور باکتری‌ها تاثیر ندارد. در تعیین غلظت موثر آب ازونیزه بر باکتری مشخص شد که غلظت 100 درصد آب ازونیزه خروجی از دستگاه قادر به گندزدایی است.

کلیدواژه‌ها

عنوان مقاله [English]

The Evaluation of Antimicrobial Effect of Ozonized Water on the Environmental Decontamination

نویسندگان [English]

  • Mohammad Reza Akbari 2
  • Sh. Faridfar 3
1
2
3
چکیده [English]

In this study, the antimicrobial effect of ozonized water was investigated in the DSM2500-SK environmental decontamination vehicle. Following a biological attack, rapid and timely decontamination would prevent the spread of contamination and reduce the extent of damage. The antimicrobial effect of ozonized water was investigated on some bacteria such as Pseudomonas aeruginosa (peripheral bacteria), Staphylococcus aureus (gram-positive bacteria), Escherichia coli (gram-negative bacteria) and Bacillus subtilis (spores), according to the existing standards. In the first step the sensitivity of bacteria was determined by the modified microdilution method by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ozonized water and in the next step the concentration of the ozonized water affecting bacteria was investigated. Rising temperatures, impurities and pH cause the instability of ozonized water. The results showed that the minimum lethal dilution of bacteria by ozonized water at a concentration of 3 ppm was 7.5×104 for Staphylococcus aureus, 7.5×105 for Pseudomonas aeruginosa, and 7.5×105 CFU/mL for Escherichia coli O157 and this disinfectant solution has no effect on bacterial spores. For the determination of the effective concentration of ozonized water on bacteria, it was found that the outlet of 100% concentrated ozonized water is capable of disinfection.

کلیدواژه‌ها [English]

  • Environmental Decontamination
  • Antimicrobial Effect
  • Ozonized Water

مراجع

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سابقه مقاله

  • تاریخ دریافت: 07 اسفند 1399
  • تاریخ بازنگری: 14 شهریور 1400
  • تاریخ پذیرش: 20 مهر 1400
  • تاریخ انتشار: 01 آبان 1400

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